Selected Publications * Corresponding author
2012
The Return of the synaptic Alpha Synuclein - When too much of a Good thing is really really bad
alpha-synuclein inhibits inter-synaptic vesicle mobility and maintains recycling pool homeostasis.
David Scott and Subhojit Roy. Journal of Neuroscience,2012 July 25; 32(30):10129-35. Read this paper Data suggest that alpha-synuclein may regulate the size of recycling pool (and consequently neurotransmitter release) by regulating vesicle-trafficking between synaptic boutons. |
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Axonal transport of alpha-synuclein
The slow axonal transport of alpha-synuclein – mechanistic commonalities amongst diverse cytosolic cargoes. Yong Tang, Utpal Das (co-first authors), David Scott and Subhojit Roy*.
Cytoskeleton (special issue on South American cell biology meeting), Jul;69(7):506-13. 2012. Read this paper Employs our new technique to study the slow axonal transport of alpha-synuclein. |
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Imaginary view of Fast and Slow Axonal Transport
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Effects of amyloid-beta on axonal transport - what's the mechanism?
Early and selective impairments in axonal transport kinetics of synaptic cargoes induced by soluble amyloid beta-protein oligomers.
Yong Tang, David Scott, Utpal Das, Steven Edland, Kryslaine Radomski, Edward H. Koo and Subhojit Roy* Traffic. May 2012;13(5):681-93
Read this paper
Describes early effects of soluble, cell-derived amyloid-beta oligomers (200 picomolar!) on axonal transport of various vesicular cargoes imaged under controlled, standardized protocols. The effects are selective (only synaptic cargoes are affected; no effects on mitochondrial motility), executed via an NMDA-receptor/GSK3b - mediated pathway, and may result from diminished cargo/motor engagement.
Yong Tang, David Scott, Utpal Das, Steven Edland, Kryslaine Radomski, Edward H. Koo and Subhojit Roy* Traffic. May 2012;13(5):681-93
Read this paper
Describes early effects of soluble, cell-derived amyloid-beta oligomers (200 picomolar!) on axonal transport of various vesicular cargoes imaged under controlled, standardized protocols. The effects are selective (only synaptic cargoes are affected; no effects on mitochondrial motility), executed via an NMDA-receptor/GSK3b - mediated pathway, and may result from diminished cargo/motor engagement.
2011
Harnessing the power of photoactivatable probes to study axonal transport - Try this at home!
A simple photoactivation and image-analysis module for visualizing and analyzing axonal transport with high temporal resolution.
Subhojit Roy*, Ge Yang, Yong Tang, and David Scott. Nature Protocols, January 2012 issue. Read this paper
Describes a simple add-on that can be retroactively fitted on an epifluorescence (Olympus) microscope to evaluate axonal transport of cargoes tagged to photoactivatable probes with high temporal resolution. A relatively low-cost option that can even be used for analyzing proteins with very fast diffusible pools, with no compromise in data-quality. Image-analyses procedures and experimental caveats/troubleshooting tips are also provided.
Subhojit Roy*, Ge Yang, Yong Tang, and David Scott. Nature Protocols, January 2012 issue. Read this paper
Describes a simple add-on that can be retroactively fitted on an epifluorescence (Olympus) microscope to evaluate axonal transport of cargoes tagged to photoactivatable probes with high temporal resolution. A relatively low-cost option that can even be used for analyzing proteins with very fast diffusible pools, with no compromise in data-quality. Image-analyses procedures and experimental caveats/troubleshooting tips are also provided.
The logic of slow axonal transport: How does the soluble protein move anyway?
Mechanistic logic underlying the axonal transport of cytosolic proteins. David Scott, Utpal Das (co-first authors), Yong Tang and Subhojit Roy*.
Neuron May 2011 12;70(3):441-54. Describes a new way by which cytosolic proteins may be transported. Read this paper
* See Preview: "The Curious Case of the Soluble Protein". Scott T. Brady. Dev Cell. 2011 May 17;20(5):581-2. Read this Preview
* See UCSD News story: “Slow Road to the Synapse: Why some proteins take their time getting there.”
Neuron May 2011 12;70(3):441-54. Describes a new way by which cytosolic proteins may be transported. Read this paper
* See Preview: "The Curious Case of the Soluble Protein". Scott T. Brady. Dev Cell. 2011 May 17;20(5):581-2. Read this Preview
* See UCSD News story: “Slow Road to the Synapse: Why some proteins take their time getting there.”
A Simulated Biopsy paradigm to hypothetically diagnose neurodegenerative diseases - A Neuropathologic study
Simulated brain biopsy for diagnosing neurodegeneration using autopsy-confirmed cases. Venneti S, Robinson JL, Roy S, White MT, Baccon J, Xie SX, Trojanowski JQ*. Acta Neuropathol. 2011 Dec;122(6):737-45. Read this paper
2010
Alpha-synuclein at the synapse: What makes a perfectly good synapse go Boink?
A pathologic cascade leading to synaptic dysfunction in α-synuclein-induced neurodegeneration. David Scott, Iustin Tabarean, Yong Tang, Anna Cartier, Eliezer Masliah and Subhojit Roy*.
Journal of Neuroscience Jun 2010 16;30(24):8083-95. Read this paper
Describes the development of a new model-system by which evolvng alpha-synuclein pathology can be quantitatively assessed in neurons.
* Editorial comment in “This week in the journal” Link to Pubmed
* UCSD News story: UCSD: News
* See report/peer-comments on the online community Alzforum: Alzforum: News
Journal of Neuroscience Jun 2010 16;30(24):8083-95. Read this paper
Describes the development of a new model-system by which evolvng alpha-synuclein pathology can be quantitatively assessed in neurons.
* Editorial comment in “This week in the journal” Link to Pubmed
* UCSD News story: UCSD: News
* See report/peer-comments on the online community Alzforum: Alzforum: News
2009
Review on axonal transport and neurodegenerative diseases
Axonal transport and neurodegenerative diseases (Review). Subhojit Roy, Virginia Lee and John Q. Trojanowski.
Encyclopedia of Neuroscience (2009), vol. 1, pp. 1199-1203.
A review of links between axonal transport and neurodegeneration. Read this paper
Encyclopedia of Neuroscience (2009), vol. 1, pp. 1199-1203.
A review of links between axonal transport and neurodegeneration. Read this paper
The paradox of alpha-synuclein
The paradoxical cell biology of α-synuclein. Roy S, Results Probl Cell Differ. 2009;48:159-72.
Alpha-synuclein is a small, soluble protein that normally localizes to the presynaptic terminals. Yet in diseased states, large amounts of alpha-synuclein is found within cell bodies and proximal neurons. This review considers the hypothesis that the axonal transport and presynaptic targeting of the protein is disturbed in diseased states. Read this paper
Alpha-synuclein is a small, soluble protein that normally localizes to the presynaptic terminals. Yet in diseased states, large amounts of alpha-synuclein is found within cell bodies and proximal neurons. This review considers the hypothesis that the axonal transport and presynaptic targeting of the protein is disturbed in diseased states. Read this paper
2008 and Before
Alpha-synuclein induces curious vesicular accumulations in the yeast
α-synuclein Induced Aggregation of Cytoplasmic Vesicles in Saccharomyces cerevisiae. James Soper, Subhojit Roy, Anna Steiber, Chris Burd, Virginia Lee.
Molecular Biology of the Cell. Jan 2008 19(3), 1093-1103.
Curious vesicular accumulations upon alpha-synuclein over-expression in yeast. Is this an artifact, or is mother nature trying to tell us something? Make up your own mind, Read this paper
Molecular Biology of the Cell. Jan 2008 19(3), 1093-1103.
Curious vesicular accumulations upon alpha-synuclein over-expression in yeast. Is this an artifact, or is mother nature trying to tell us something? Make up your own mind, Read this paper
Role of f-actin on transport of cytosolic cargoes
Cytoskeletal requirements of Slow Component-b transport. Subhojit Roy, Matthew J. Winton, Mark M. Black, John Q. Trojanowski, Virginia Lee.
Journal of Neuroscience May 2008 28(20):5248-5256. Read this paper
Journal of Neuroscience May 2008 28(20):5248-5256. Read this paper
Co-transport of cytosolic cargoes
Rapid and intermittent co-transport of Slow Component-b proteins. Subhojit Roy, Matthew J. Winton, Mark M. Black, John Q. Trojanowski, Virginia Lee.
Journal of Neuroscience Mar 2007 27(12):3131-38. Read this paper
* Featured with editorial comment in “This week in the journal”
Journal of Neuroscience Mar 2007 27(12):3131-38. Read this paper
* Featured with editorial comment in “This week in the journal”
Axonal transport of neurofilament polymers: The one that started it all
Neurofilaments are Transported Rapidly but Intermittently in Axons: Implications for Slow Axonal Transport. Subhojit Roy, Pilar Coffee, George Smith, Ron K. Liem, Scott T. Brady and Mark M. Black.
Journal of Neuroscience Sept 2000; 20(18):6849-6861. Read this paper
Subhojit Roy's PhD thesis!
Journal of Neuroscience Sept 2000; 20(18):6849-6861. Read this paper
Subhojit Roy's PhD thesis!
